Which of the following is not a reagent required in an enzymatic serum glucose method?

Which of the following is not a reagent required in an enzymatic serum glucose method?

• A. NAD+
• B. Glucose oxidase
• C. Peroxidase
• D. Reduced chromogen

Answer: (A)

In the common enzymatic serum glucose assay, glucose oxidase oxidizes glucose to gluconic acid and generates hydrogen peroxide. Horseradish peroxidase then uses that hydrogen peroxide to oxidize a reduced chromogen, producing a colored product whose intensity corresponds to the glucose level. The reagents you actually add in this method are glucose oxidase, peroxidase, and the reduced chromogen. NAD+ is not required here; it is used in alternative glucose assays that rely on glucose dehydrogenase, where NAD+ serves as an electron acceptor. So NAD+ isn’t part of the standard GOD-POD enzymatic procedure.

In the common enzymatic serum glucose assay, glucose oxidase oxidizes glucose to gluconic acid and generates hydrogen peroxide. Horseradish peroxidase then uses that hydrogen peroxide to oxidize a reduced chromogen, producing a colored product whose intensity corresponds to the glucose level. The reagents you actually add in this method are glucose oxidase, peroxidase, and the reduced chromogen. NAD+ is not required here; it is used in alternative glucose assays that rely on glucose dehydrogenase, where NAD+ serves as an electron acceptor. So NAD+ isn’t part of the standard GOD-POD enzymatic procedure.